ournal of Medicine and Medical Research  ISSN 2315-943X  Vol. 4(1), pp. 1-7,  July 2016

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  Full Length Research

Comparison of a rapid diagnostic (SD bioline malaria Ag p.f/pan) test kit with microscopy for detection of malaria parasites among blood donors in Kaduna state, Nigeria.

 D.D. Garba1*, M. Aminu2, J.B. Ameh2 and C.M.Z, Whong2

*Corresponding author. E-mail: Tel. 08023634977

School of Medical Laboratory Sciences, Ahmadu Bello University Teaching Hospital, Zaria, Kaduna State, Nigeria.


.Accepted 12 July, 2016                 


The screening of pre-transfusion blood donors for malarial parasitaemia to reduce the risk of transmission transmitted malaria is of paramount importance in efforts to enhance the safety of donor blood and control of morbidity and mortality caused by malaria in malaria endemic areas. This study evaluated the diagnostic performance of a rapid diagnostic test (RDT) for detection of histidine-rich protein II (HRP-II) of Plasmodium falciparum and Plasmodium lactate dehydrogenase forthe other Plasmodium species in the peripheral blood of 360 asymptomatic blood donors reporting at the blood transfusion in three secondary health facilities of Kaduna State, Nigeria for blood donation. Microscopic examination of Giemsa stained blood smears was used as the gold standard. Results showed that 27(7.5%) of the donors were parasitaemic by microscopy whereas 31(8.6%) were positive by RDT. Ten (10) of the donors were negative by microscopy but positive by RDT (false positive) while 6 were negative by RDT but positive by microscopy (false negative). Thorough microscopic examination by two experienced microscopists revealed that P. falciparum accounted for all (100%) the positive cases. There were no mixed infections and no other blood parasites were encountered. Further analysis of the results showed a sensitivity of 77.8%, specificity of 97%, positive predictive value of 67.7% and negative predictive value of 98.2%. RDT was substantially correlated (κ = 0.700) with the reference method. The RDT is a valuable tool for screening of blood donors and epidemiological studies. The cost of most of these RDTs remains a major obstacle to widespread use in areas of malaria endemicity.

Key words: Comparison,diagnostic,microscopy, malaria parasite, blood donors.

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